[o-info] question on resolution

From: Michael Colaneri (colanerim@gmail.com)
Date: Thu Mar 01 2007 - 18:54:18 CST


Hello,

I have two questions:

(1)
Does the total number of hydrogen bonds in a structure (or # of
contacts <4A or # of salt bridges -- whatever is usually determined
based on a threshold) depend on resolution?  I would think that it is
even in the best structure at 3 A because the side chains are
determined from rotamers etc, there is more luzzati error and more
errors because we do not see the side chains as clearly as at high
res.  Is there a systematic study published on this?  I have not seen
any.

(2)
Does packing density of a protein defined as Van der Waals volume over
actual volume (Voronoi polyhedra) depend on resolution?  I would think
not.  Does it depend on errors in structure?  I would think again no.

I would appreciate your opinions in these two questions and if you
have any references.

Thanks.

Mike Colaneri
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