[o-info] peptide connectivity

From: Karen Champagne (ksc1130@yahoo.com)
Date: Tue Feb 13 2007 - 17:21:17 CST


Hi All,

I am having some trouble with a peptide ligand bound
to my protein.  It is an 8-residue ligand and one of
the residues is post-translationally modified. 
Because of this, when I load the model into O none of
the residues in the ligand are connected (i.e. no
peptide bonds).  I am wondering how I can tell O to
connect all the residues?  If I do 'make bond' between
them all it is fine at first, but after a round of
refinement the bonds are lost again when I load the
new model.

Thanks in advance!

kc


 
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