Does anyone have a suggestion what can be done in the case of high B factors in X-PLOR 3.851? I am not sure but it seems like a refinement algorithm artifact and I bet the referees won't like this excuse. The 30-2.5A cryo data is complete, strong and bulk solvent corrected and the density and Rfree are v.good. I tried different WA, tried overall, group and individual B refinement and if I use a few cycles, just until the Rfree stops dropping fast, the B's are going up to an average of 55.00 for all protein, ligands and solvent atoms. Anyone ? Please... Gitay
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