> loops near the active site of an enzyme cause a more serious problem. Again > complete 1.5A data or better would probably be convincing but what if you > have 2.2A or 2.6A? Yes. I think there are a number of schools of thought on what the best approach is. If you have weak density, then contouring maps at lower levels can help. If you have biochemical/mutation data which corroborates your model, that would help convince a referee. Refinement with REFMAC (CCP4) using maximum likelihood refinement can clarify weak density. Aaron
This archive was generated by hypermail 2b29 : Tue Mar 20 2007 - 03:13:21 CST