Recipe for adding residues to the Mutate_replace repertoire

From: Gerard 'CD' Kleywegt (GERARD@XRAY.BMC.UU.SE)
Date: Tue Feb 25 1997 - 14:20:41 CST


to use new residue types in mut_* commands,you need to add residues
to the rotamer database:

- s_a_i a little pdb file with only your new residue

- call the molecule SCxxx, where "xxx" is the three-character residue
  type (ALA, GLY, ...)

- let's assume you only have one rotamer to keep it simple

- write all the datablocks related to your SCxxx molecule to a file:
  O> write scxxx_* scxxx.odb ;

- edit this file to only keep the following datablocks:  _ATOM_XYZ
  _ATOM_B  _RESIDUE_NAME  _RESIDUE_TYPE  _ATOM_NAME  _RESIDUE_POINTERS
  _RESIDUE_CG  _ATOMS_IN_RESIDUES
  (the latter one you probably have to create yourself; see the
  $ODAT/rsc.o file for examples)

- add these datablocks to your (own copy of) $ODAT/rsc.o

- if all went well, you should now be able to mut_replace any residue by
  the new type you just defined

if your local rsc.o file is binary, then:

(1) either read rsc.o then write all sc* datablocks to an ascii file

(2) or (better): get file pub/o/newer_rsc.o from rose.bmc.uu.se (updated
    set of rotamers; ascii file)

--cd



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