1. Thaw frozen (-80 ° C) competent cells on ice
   2. Mix 1-10 µl DNA with 40-80 µl cells
   3. Incubate on ice 20 min
   4. Incubate 42 ° C 45 sec
   5. Incubate on ice 2 min
   6. Add 1 ml LB (proportional to 40-80 µl cells)
   7. Shake 37 ° C 45 min
   8. (Optional) Spin cells down (8 sec pressing button) to reduce volume 
       (Decanting most but not all of supernatant may increase yield if there are a low # colonies)
   9. Plate 50-100 µl cells LB(antibiotic)agar plate