1. Spin down 1.5 ml freshly saturated culture in microfuge, decant supernatant
2. Resuspend cells by vortexing in 300 µl STET
3. Add 20 µl of 50 mM Tris pH 8.0 that contains 10 mg/ml lysozyme
4. Mix and incubate at room temperature, 0.5-10 min.
5. Place in boiling water bath, 2 min
6. Spin in microfuge (14,000 rpm), 5 min
7. Scoop out and discard "snot" with a toothpick
8. Add 325 µl of 75% isopropanol with 2.5 M NH4OAc
9. Mix and microfuge, 5 min
10. Discard supernatant
11. Rinse pellet wih 70% EtOH (pellet is spread over wall of tube)
12. Mix and microfuge, 5 min
13. Carefully draw off most (~95%) supernatant leaving pellet in bottom of tube
14. Lyophilize, 5-10 min
15. Resuspend in 50 µl TE with RNase

One tube, clean high yield plasmid DNA prep with no phenol

Solution	Storage Temp
STET = 8% Sucrose, 5% Triton X-100, 50 mM EDTA, 50 mM Tris 8.0	RT
75% isopropanol, 2.5 M NH4OAc = 3:1 isopropanol:10 M NH4OAc	RT
70% EtOH	RT
50 mM Tris pH 8.0 with 10 mg/ml lysozyme	-20 C